hybridization solution (Cytiva Europe)
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Hybridization Solution, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 116 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+solution/Microarray+Hybridization+Solution+v2%2E0/ppr0512960-248-8-20
Average 93 stars, based on 116 article reviews
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Comparison:Article Title: DISTINCTIVE TRANSCRIPTOME RESPONSE OF TWO STRAWBERRY (FRAGARIA × ANANASSA) CULTIVARS TO COLLETOTRICHUM ACUTATUM INFECTION Article Snippet: Strawberry (Fragaria × ananassa) yields are strongly affected by fungal diseases and pests, e.g., Colletotrichum spp., forcing the excessive use of chemical products (mostly environmental contaminants) to control them.. Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberry’s resistance to pathogens, and completely resistant cultivars have not been reported.. Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes. Infection:Article Title: DISTINCTIVE TRANSCRIPTOME RESPONSE OF TWO STRAWBERRY (FRAGARIA × ANANASSA) CULTIVARS TO COLLETOTRICHUM ACUTATUM INFECTION Article Snippet: Strawberry (Fragaria × ananassa) yields are strongly affected by fungal diseases and pests, e.g., Colletotrichum spp., forcing the excessive use of chemical products (mostly environmental contaminants) to control them.. Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberry’s resistance to pathogens, and completely resistant cultivars have not been reported.. Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes. Labeling:Article Title: DISTINCTIVE TRANSCRIPTOME RESPONSE OF TWO STRAWBERRY (FRAGARIA × ANANASSA) CULTIVARS TO COLLETOTRICHUM ACUTATUM INFECTION Article Snippet: Strawberry (Fragaria × ananassa) yields are strongly affected by fungal diseases and pests, e.g., Colletotrichum spp., forcing the excessive use of chemical products (mostly environmental contaminants) to control them.. Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberry’s resistance to pathogens, and completely resistant cultivars have not been reported.. Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes. Article Title: A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine Article Snippet: .. Finally, 25 μL 2× GE Healthcare hybridization buffer was added to dilute the labeled cRNA, and 50 μL of Article Title: A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum Article Snippet: Proteinase K (4.55 U/ml; Roche Applied Science, Indianapolis, IN) was added to the buffer, and the mixture was incubated for 6 min, followed by 4 min of subjection to a continuous vortex with glass beads (Sigma, St. Louis, MO) at room temperature; the RNeasy mini kit was used according to the instructions of the manufacturer (QIAGEN, Valencia, CA), and genomic DNA contamination was minimized by incubating buffer RW1 ( 1 ) at room temperature for 4 min; isolated RNA was eluted in 30 to 40 μl of RNase-free water. .. RNA samples for Article Title: A chemical defense deters cannibalism in migratory locusts Article Snippet: .. Subsequently, each slide was covered with 130 μl Article Title: A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum Article Snippet: Proteinase K (4.55 U/ml; Roche Applied Science, Indianapolis, IN) was added to the buffer, and the mixture was incubated for 6 min, followed by 4 min of subjection to a continuous vortex with glass beads (Sigma, St. Louis, MO) at room temperature; the RNeasy mini kit was used according to the instructions of the manufacturer (QIAGEN, Valencia, CA), and genomic DNA contamination was minimized by incubating buffer RW1 (1) at room temperature for 4 min; isolated RNA was eluted in 30 to 40 l of RNase-free water. .. RNA samples for Hybridization:Article Title: DISTINCTIVE TRANSCRIPTOME RESPONSE OF TWO STRAWBERRY (FRAGARIA × ANANASSA) CULTIVARS TO COLLETOTRICHUM ACUTATUM INFECTION Article Snippet: Strawberry (Fragaria × ananassa) yields are strongly affected by fungal diseases and pests, e.g., Colletotrichum spp., forcing the excessive use of chemical products (mostly environmental contaminants) to control them.. Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberry’s resistance to pathogens, and completely resistant cultivars have not been reported.. Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes. Article Title: A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine Article Snippet: .. Finally, 25 μL 2× GE Healthcare hybridization buffer was added to dilute the labeled cRNA, and 50 μL of Article Title: A chemical defense deters cannibalism in migratory locusts Article Snippet: .. Subsequently, each slide was covered with 130 μl Article Title: Thyroid Hormone Deficiency Affects Postnatal Spiking Activity and Expression of Ca 2+ and K + Channels in Rodent Inner Hair Cells Article Snippet: .. Briefly, DIG-labeled antisense or sense probes were diluted in Article Title: Receptor guanylyl cyclase-G is a novel thermosensory protein activated by cool temperatures Article Snippet: A digoxigenin-labeled antisense riboprobe was generated from a partial cDNA clone in a pGem-T plasmid encoding mouse c-Fos or GC-G by use of the T7/SP6 RNA transcription system (Roche Diagnostics, Mannheim, Germany) as recommended by the manufacturer. .. After fixation in 4% paraformaldehyde/0.1 M NaHCO 3 /pH 9.5 for 45 min at 4°C, slices were washed in 1× PBS (0.85% NaCl, 1.4 mM KH 2 PO 4 , 8 mM Na 2 HPO 4 , pH 7.4) for 1 min at room temperature, then incubated in 0.2 M HCl for 10 min, in 1% Triton X-100/1× PBS for 2 min and washed twice in 1× PBS for 30 s. Finally, sections were incubated in 50% formamide/5× SSC (0.75 M NaCl, 0.075 M sodium citrate, pH 7.0) for 10 min. Then, tissue was hybridized in Article Title: Expression of cGMP signaling elements in the Grueneberg ganglion. Article Snippet: The Grueneberg ganglion (GG) is a cluster of neurons localized to the vestibule of the anterior nasal cavity.. Based on axonal projections to the olfactory bulb of the brain, as well as expression of olfactory receptors and the olfactory marker protein, it is considered a chemosensory subsystem.. Recently, it was observed that in mice, GG neurons respond to cool ambient temperatures. Microarray:Article Title: DISTINCTIVE TRANSCRIPTOME RESPONSE OF TWO STRAWBERRY (FRAGARIA × ANANASSA) CULTIVARS TO COLLETOTRICHUM ACUTATUM INFECTION Article Snippet: Strawberry (Fragaria × ananassa) yields are strongly affected by fungal diseases and pests, e.g., Colletotrichum spp., forcing the excessive use of chemical products (mostly environmental contaminants) to control them.. Resistant cultivars are a priority of most strawberry breeding programs; however, little is known about the genetic basis for strawberry’s resistance to pathogens, and completely resistant cultivars have not been reported.. Although molecular markers of disease resistance in strawberry have been reported, the octoploid genetic structure of commercial strawberry makes it difficult to associate molecular markers with disease resistance genes. Article Title: A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine Article Snippet: .. Finally, 25 μL 2× GE Healthcare hybridization buffer was added to dilute the labeled cRNA, and 50 μL of Article Title: A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum Article Snippet: Proteinase K (4.55 U/ml; Roche Applied Science, Indianapolis, IN) was added to the buffer, and the mixture was incubated for 6 min, followed by 4 min of subjection to a continuous vortex with glass beads (Sigma, St. Louis, MO) at room temperature; the RNeasy mini kit was used according to the instructions of the manufacturer (QIAGEN, Valencia, CA), and genomic DNA contamination was minimized by incubating buffer RW1 ( 1 ) at room temperature for 4 min; isolated RNA was eluted in 30 to 40 μl of RNase-free water. .. RNA samples for Article Title: A chemical defense deters cannibalism in migratory locusts Article Snippet: .. Subsequently, each slide was covered with 130 μl Article Title: Thyroid Hormone Deficiency Affects Postnatal Spiking Activity and Expression of Ca 2+ and K + Channels in Rodent Inner Hair Cells Article Snippet: .. Briefly, DIG-labeled antisense or sense probes were diluted in Article Title: Receptor guanylyl cyclase-G is a novel thermosensory protein activated by cool temperatures Article Snippet: A digoxigenin-labeled antisense riboprobe was generated from a partial cDNA clone in a pGem-T plasmid encoding mouse c-Fos or GC-G by use of the T7/SP6 RNA transcription system (Roche Diagnostics, Mannheim, Germany) as recommended by the manufacturer. .. After fixation in 4% paraformaldehyde/0.1 M NaHCO 3 /pH 9.5 for 45 min at 4°C, slices were washed in 1× PBS (0.85% NaCl, 1.4 mM KH 2 PO 4 , 8 mM Na 2 HPO 4 , pH 7.4) for 1 min at room temperature, then incubated in 0.2 M HCl for 10 min, in 1% Triton X-100/1× PBS for 2 min and washed twice in 1× PBS for 30 s. Finally, sections were incubated in 50% formamide/5× SSC (0.75 M NaCl, 0.075 M sodium citrate, pH 7.0) for 10 min. Then, tissue was hybridized in Article Title: Expression of cGMP signaling elements in the Grueneberg ganglion. Article Snippet: The Grueneberg ganglion (GG) is a cluster of neurons localized to the vestibule of the anterior nasal cavity.. Based on axonal projections to the olfactory bulb of the brain, as well as expression of olfactory receptors and the olfactory marker protein, it is considered a chemosensory subsystem.. Recently, it was observed that in mice, GG neurons respond to cool ambient temperatures. Article Title: A General Framework for Designing and Validating Oligomer-Based DNA Microarrays and Its Application to Clostridium acetobutylicum Article Snippet: Proteinase K (4.55 U/ml; Roche Applied Science, Indianapolis, IN) was added to the buffer, and the mixture was incubated for 6 min, followed by 4 min of subjection to a continuous vortex with glass beads (Sigma, St. Louis, MO) at room temperature; the RNeasy mini kit was used according to the instructions of the manufacturer (QIAGEN, Valencia, CA), and genomic DNA contamination was minimized by incubating buffer RW1 (1) at room temperature for 4 min; isolated RNA was eluted in 30 to 40 l of RNase-free water. .. RNA samples for Expressing:Article Title: A Transcriptome-Level Study Identifies Changing Expression Profiles for Ossification of the Ligamentum Flavum of the Spine Article Snippet: .. Finally, 25 μL 2× GE Healthcare hybridization buffer was added to dilute the labeled cRNA, and 50 μL of Incubation:Article Title: Receptor guanylyl cyclase-G is a novel thermosensory protein activated by cool temperatures Article Snippet: A digoxigenin-labeled antisense riboprobe was generated from a partial cDNA clone in a pGem-T plasmid encoding mouse c-Fos or GC-G by use of the T7/SP6 RNA transcription system (Roche Diagnostics, Mannheim, Germany) as recommended by the manufacturer. .. After fixation in 4% paraformaldehyde/0.1 M NaHCO 3 /pH 9.5 for 45 min at 4°C, slices were washed in 1× PBS (0.85% NaCl, 1.4 mM KH 2 PO 4 , 8 mM Na 2 HPO 4 , pH 7.4) for 1 min at room temperature, then incubated in 0.2 M HCl for 10 min, in 1% Triton X-100/1× PBS for 2 min and washed twice in 1× PBS for 30 s. Finally, sections were incubated in 50% formamide/5× SSC (0.75 M NaCl, 0.075 M sodium citrate, pH 7.0) for 10 min. Then, tissue was hybridized in Article Title: Expression of cGMP signaling elements in the Grueneberg ganglion. Article Snippet: The Grueneberg ganglion (GG) is a cluster of neurons localized to the vestibule of the anterior nasal cavity.. Based on axonal projections to the olfactory bulb of the brain, as well as expression of olfactory receptors and the olfactory marker protein, it is considered a chemosensory subsystem.. Recently, it was observed that in mice, GG neurons respond to cool ambient temperatures. |
